Thermo Fisher, 34060, IPTG

Quantity: 1 g
Unit Size: Each
X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside) is a chromogenic substrate forβ-Galactosidase. It yields a blue precipitate, is soluble in dimethylformamide, and can be used to detect vectors, plasmids or DNA fragments that contain the β-Galactosidase gene. Highlights:
• See item-specific features X-Gal (5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside)
• Chromogenic substrate for β-Galactosidase
• Yields a blue precipitate
• Soluble in DMF
• Can be used to detect vectors, plasmids, or DNA fragments that contain the β-Galactosidase gene IPTG (Isopropyl-β-D-thiogalactopyranoside)
• Maximizes the expression of genes in expression vectors
• When screening a lambda bacteriophage library with an antibody, the probability of success can be increased by preventing the expression of the fusion protein until the plaques are well established
• By placing a nitrocellulose filter containing the inducer IPTG onto the plate and continuing growth at 37°C, the expression of the potential β-galactosidase-cDNA fusion protein can be induced after three to four hours of plaque growth; the nitrocellulose filter is then screened with antibodies
• IPTG is also used in combination with X-Gal to detect active β-galactosidase
• Practical in applications using Bacteriophage M13 and Igt11 vectors