Thermo Fisher, 38042024, Topoisomerase I

Name: Topoisomerase I
Brand: Thermo Fisher
SKU: 38042024

Quantity: 500 U, Unit Size: Each

Catalog Number: 38042024

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Category

Phosphatase Inhibitors

Pack Size1 unit / pack

TagsModifying Enzymes Enzymes and Inhibitors

CollectionsThermo Fisher Enzymes & Inhibitors for PCR, Cloning & Protein Protection Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 500 U
Unit Size: Each
• Topoisomerase I is active in the presence of EDTA
• Source: Purified from calf thymus
• Performance and Quality Testing: Endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease, and phosphatase assays; conversion of super-coiled DNA to relaxed DNA
• Unit Definition: One unit catalyzes the conversion of 0.5μg of superhelical ΦX174 RF DNA to a relaxed state in 30 min. at 37°C
• Unit Reaction Conditions: 50mM Tris-HCl (pH 7.5), 50mM KCl, 10mM MgCl_2, 0.1mM EDTA, 0.5mM DTT, 30 μg/mL BSA, 0.5μg ΦX174 RF DNA, and enzyme in 50μL for 30 min. at 37°CRelaxing positively and negatively supercoiled DNA, Producing DNA topoisomers, Cloning, Restriction Enzyme Cloning

Specifications

General

Product Type	Topoisomerase I
Quantity	500 U
Shipping Condition	Wet Ice
Enzyme	Topoisomerase
Unit Size	Each

Ordering

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Thermo Fisher, 38042024, Topoisomerase I

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