Thermo Fisher, 78200.200.UL, ExoSAP-IT™ PCR Product Cleanup Reagent, 200 μL

ExoSAP-IT™ PCR Product Cleanup Reagent provides one-step enzymatic purification of amplified PCR products before sequencing or SNP analysis. The reagent removes residual primers and unincorporated nucleotides directly in the original PCR vessel, avoiding column transfers and helping preserve the amplified DNA. Cleanup uses two incubations: the first digests excess primers and dephosphorylates free nucleotides, while the second heat step inactivates the cleanup enzymes. After treatment, the amplicon can proceed directly to downstream sequencing workflows without an additional purification step. This 200 µL configuration supports 100 reactions. The single-addition format is useful for routine Sanger sequencing preparation where reduced handling, lower risk of cross-contamination, and high amplicon recovery are priorities. Ship on dry ice and store at −20 °C.