Thermo Fisher, 88-18005-204, PrimeFlow™ RNA Assay Kit, 40 Tests

PrimeFlow™ RNA Assay Kit, 40 Tests is designed for simultaneous measurement of RNA transcripts and immunophenotyping markers at single-cell resolution by flow cytometry. This 40-test PrimeFlow RNA format uses in situ hybridization based on branched-DNA signal amplification to detect up to four RNA targets while retaining compatibility with fluorescent antibody staining for cell-surface or intracellular proteins. Pairs of target-specific probes bind the RNA transcript, and branched-DNA amplification increases the detectable signal by approximately 8000- to 16,000-fold. The method allows RNA expression to be associated with protein-marker phenotypes within individual cells rather than relying on bulk RNA measurements. The assay kit includes reagents for fixation, permeabilization, target hybridization, preamplification, amplification and labeling, with materials supplied for 40 tests. The workflow is suited to immunology, cell-population characterization and studies in which gene expression needs to be resolved among phenotypically distinct cell subsets. Detection uses a compatible flow cytometer rather than a standard plate-based fluorescence assay. Store kit components under the conditions assigned to each reagent, including the refrigerated components identified for the 40-test package. This is an RNA detection and cellular phenotyping workflow, not a nucleic-acid extraction kit. RNA targets can be related to cellular phenotypes within the same specimen because hybridization signals and antibody fluorescence are measured together. The 40-test package supports experiments that require this combined transcript-and-protein readout.