Description
Quantity: 1200 U
Concentration: 40 U/μL
Unit Size: Each
Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors. By retaining strong strand-displacement activity, this enzyme helps enable highly efficient isothermal amplifications such as loop-mediated isothermal amplification (LAMP).Lyo-ready Bst DNA Polymerase possesses 5´→3´ DNA polymerase activity and lacks 5´→3´ exonuclease activity and 3´→5´ exonuclease activity. It is also a glycerol-free enzyme, enabling assay lyophilization and use in microfluidics-based technologies.Lyo-ready Bst DNA Polymerase features• Fast target detection in as little as 10 minutes• Enhanced sensitivity down to 50 copies using optimized reaction buffer in (RT-)LAMP reaction• Robust amplification, even from inhibitor-containing RNA or DNA samples• Ability to optimize LAMP or RT-LAMP reactions to fit your needsApplicationsLyo-ready Bst DNA Polymerase can be used for a wide variety of applications including:• Loop-mediated isothermal amplification (LAMP)• Whole genome amplification (WGA)• Rolling circle amplification (RCA)• Recombinase polymerase amplification (RPA)• Helicase-dependent amplification (HDA)• Exponential amplification reaction (EXPAR)Notes1. If performing real-time LAMP detection with SYTO 9 Green Fluorescent Nucleic Acid Stain, dilute the stock to 50 μM with nuclease-free water before every use.2. SuperScript IV Reverse Transcriptase and RNaseOUT Recombinant Ribonuclease Inhibitor are recommended for reactions containing RNA targets.
