Thermo Fisher, A72125, Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)

Quantity: 120 μg
Product Type: Lyo-ready T4 UvsY Protein
Unit Size: Each
Features of Lyo-ready RPA products
• Enhanced sensitivity down to 1 copy using optimized (RT-)RPA reaction buffer
• Robust and specific RNA/DNA amplification
• Amplification from inhibitor-containing samples
• Fast target amplification in as little as 20 minutes
• Ability to easily optimize RPA or RT-RPA reactionsKit componentsThe Lyo-ready RPA Kit includes all required components for an RPA reaction:
• Lyo-ready T4 UvsX Protein—produced fromE. colicells bearing cloned uvsX gene derived from bacteriophage T4
• Lyo-ready T4 UvsY Protein—a recombination mediator protein that stimulates homologous recombination performed by the UvsX recombinase
• Lyo-ready Bst DNA Polymerase (Cat. No. A56655)—a proprietary mutant of Bst DNA Polymerase, Large Fragment, with fast reaction speed, increased sensitivity, and tolerance to inhibitors
• Lyo-ready T4 Gene 32 Protein—a single-stranded DNA binding protein produced fromE. colicells carrying cloned bacteriophage T4 gene 32
• Optimized reaction buffer, magnesium chloride, and dNTPs