Thermo Fisher, AM1926, RiboPure™ RNA Purification Kit, yeast

Quantity: 50 Preps
Unit Size: Each
For the isolation of extremely high-quality RNA from yeast without the use of enzymatic pretreatments that may alter gene expression profiles. Each Invitrogen kit includes sufficient reagents for 50 purifications each from 10^7 to 10^8 yeast cells.It has traditionally been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Many protocols use enzymatic pretreatments that may affect gene expression patterns. The RiboPure-Yeast RNA Isolation Kit uses no enzymatic pretreatments and thus does not alter expression profiles. It combines a simple and efficient glass bead/one step phenol–mediated disruption using Invitrogen's Vortex Bead Beater Adapter followed by a glass fiber filter– based RNA purification step. After DNase treatment with the included DNA-free™ Reagents, the resulting RNA is exceptionally pure containing little to no genomic DNA (less than 0.05%). The kit has been used extensively at Invitrogen to isolate high-quality RNA from Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris, and Candida albicans. The kit provides the highest yield of any method tested from each of these species.
• Highest yields of total RNA available
• No enzymatic pretreatment required
• Includes DNA-free™ ReagentsNot high-throughput compatible (Manual)Applications:DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Yeast