Thermo Fisher, AM2544, Proteinase K Powder, 500 mg

Name: Proteinase K Powder, 500 mg
Brand: Thermo Fisher
SKU: AM2544

Quantity: 500 mg, Unit Size: Each

Catalog Number: AM2544

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Category

Protease Inhibitors

Pack Size500 mg

TagsModifying Enzymes Enzymes and Inhibitors

CollectionsThermo Fisher Enzymes & Inhibitors for PCR, Cloning & Protein Protection Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 500 mg
Unit Size: Each
Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins.Proteinase K Remains Active:• Over a wide pH range (optimal activity between 6.5 and 9.5)• Under denaturing conditions (e.g. the presence of SDS or urea)• In the presence of metal chelating agents (e.g. EDTA)• At comparatively high temperatures (optimum digestion temperature is 65°C)Applications: Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.Performance and Quality Testing: Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.Unit Definition: One mAnson unit is described as that amount of enzyme that liberates 1 μmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.

Specifications

General

Host Species	T. album
Packaging	Bottle
Product Type	Proteinase K
Quantity	500 mg
Shipping Condition	Dry Ice
Product Line	Ambion
Unit Size	Each

FAQs

Question: What pack size and form are supplied?

Answer:500 mg of Proteinase K in dry powdered form.

Question: What is Proteinase K used for?

Answer:Broad protein digestion during DNA/RNA preparation, nuclease removal and compatible tissue-processing workflows.

Question: How should the powder be stored?

Answer:Store the dry powder with desiccant at −20 °C, following the reagent handling guidance.

Application Note

Invitrogen Ambion Proteinase K powder is a broad-specificity serine protease used to digest proteins during nucleic-acid purification, tissue processing and other molecular biology sample-preparation workflows. The supplied amount is 500 mg of powdered enzyme derived from the fungus Engyodontium album. Proteinase K remains useful under many protein-denaturing conditions, including protocols with SDS, urea or EDTA, making it suitable for inactivation and digestion of sample proteins that might otherwise interfere with DNA or RNA recovery. The stated optimum pH range is approximately 6.5–9.5, and its optimum digestion temperature is about 65 °C; actual incubation settings must follow the chosen protocol and sample type. Powder should be stored dry with desiccant at −20 °C. The reagent is an active protease and should be handled to avoid contamination, exposure and unintended degradation of sample proteins.

Ordering

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