Thermo Fisher, AM2694, SUPERase·In™ RNase Inhibitor (20 U/μL), 2500 U

Name: SUPERase·In™ RNase Inhibitor (20 U/μL), 2500 U
Brand: Thermo Fisher
SKU: AM2694

Quantity: 2500 U, Unit Size: Each

Catalog Number: AM2694

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Category

Inhibitors & Activators

TagsMolecular Biology Reagents and Kits RNA Preparation and Purification

CollectionsThermo Fisher Molecular Biology Reagents & Kits Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 2500 U
Unit Size: Each
SUPERase·In™ RNase Inhibitor is a protein-based inhibitor of nonhuman origin that noncovalently binds and inhibits the most common and troublesome RNases, including RNase A, B, C, 1, and T1. SUPERase·In RNase Inhibitor can be used in any application where RNase contamination could be problematic. Because it inhibits a broader range of RNases than traditional RNase inhibitors, SUPERase·In is the most effective RNase inhibitor available, providing a higher level of protection against degradation. SUPERase·In does not interfere with other enzymes such as RNA polymerases, reverse transcriptase, or Taq DNA polymerase. Additionally, SUPERase In is active up to 65°C and over a pH range of 5.5 to 8.5.
• Completely removes RNase contamination from glass and plastic surfaces
• Excels at removing high levels of RNase contamination where similar products fail
• Proven effective at removing high concentrations of dried-on RNase A
• Ideal for cleaning work surfaces, pipettors, and equipment that must be RNase-freeUnit definitionSUPERase·In RNase Inhibitor at 1U/μL will block the degradation of 0.1μg/μL labeled RNA by 2.5pg/μL of RNase A, 2.5pg/μL of RNase I, and 0.0075U/μL of RNase T1, for four hours at 37°C, in 20mM Tris-HCl, pH 7.5, 50mM NaCl, 1mM EDTA. Analysis is by denaturing PAGE. SUPERase·In is currently the only ribonuclease inhibitor for which the unit activity is defined by such a functional assay.If using this product with standard antibody purification methods, please contact technical support to discuss experimental design.Gene Expression Analysis and Genotyping, In Vitro Transcription, PCR and Real-Time PCR, Real Time PCR (qPCR), Reverse Transcription

Specifications

General

Concentration	50 mM
Product Line	Ambion, SUPERase•In
Quantity	2500 U
Shipping Condition	Dry Ice
Unit Size	Each

FAQs

Question: Which RNases can SUPERase·In inhibit?

Answer:It inhibits several common RNases, including RNase A, B, C, 1 and T1.

Question: What concentration is supplied?

Answer:The inhibitor is supplied at 20 U/µL, with 2500 total units.

Question: Can it be used with reverse transcriptase or Taq polymerase?

Answer:Yes. It does not interfere with reverse transcriptase, Taq DNA polymerase or RNA polymerases under suitable reaction conditions.

Application Note

SUPERase·In™ RNase Inhibitor is a non-human protein inhibitor used to protect RNA from degradation by common ribonucleases. It inhibits RNase A, B, C, 1 and T1 and can be added to workflows where accidental RNase contamination could compromise RNA integrity. The reagent does not interfere with enzymes such as RNA polymerases, reverse transcriptase or Taq DNA polymerase, making it suitable for many RNA-handling and enzymatic procedures. Activity is maintained up to 65 °C across approximately pH 5.5–8.5. This configuration contains 2500 units at 20 U/µL and is supplied in a glycerol-containing storage buffer for frozen storage. Typical uses include RNA isolation, reverse transcription, in vitro transcription, RNA labeling and other molecular-biology procedures that require additional protection against RNase-mediated degradation. Its broad RNase inhibition profile is useful when sample history is uncertain or several handling steps create repeated opportunities for environmental RNase exposure.

Ordering

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