Thermo Fisher, B52, TBE Buffer (Tris-borate-EDTA) (10X)

Quantity: 1 L
Unit Size: Each
Usage Recommendations
• Use fresh 1X TBE both for the gel and for the electrophoresis run
• To prepare 1X TBE buffer, add 100mL of 10X TBE buffer to 900mL of deionized water and mix wellQuality Control
• The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests1X Composition
• 89mM Tris, 89mM boric acid, 2mM EDTA, pH of 10X TBE: 8.3 Recommended for:Electrophoresis of nucleic acids in agarose and polyacrylamide gels; Used both as a running buffer and as a gel preparation buffer; Filtered through a 0.22μm membrane; Recommended for electrophoresis of RNA and DNA fragme. Note:Double-stranded linear nucleic acid molecules migrate about 10% slower in TBE buffer than in TAE buffer.