Thermo Fisher, EL0016, T4 DNA Ligase, LC (1 U/μL)

Name: T4 DNA Ligase, LC (1 U/μL)
Brand: Thermo Fisher
SKU: EL0016

Quantity: 2 x 500 units, Unit Size: Each, Catalyze the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA with the Thermo Scientific™ T4 DNA Ligase. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.The T4 DNA Ligase requires ATP as a cofactor.

Catalog Number: EL0016

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Category

Restriction Enzymes

Pack Size500 units / pack

TagsModifying Enzymes Enzymes and Inhibitors

CollectionsThermo Fisher Enzymes & Inhibitors for PCR, Cloning & Protein Protection Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 2 x 500 units
Unit Size: Each
Highlights
• Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP)
• Fast – sticky-end ligation is completed in 10 minutes at room temperature
• Supplied with PEG solution for efficient blunt-end ligationApplications
• Cloning of restriction enzyme generated DNA fragments
• Cloning of PCR products
• Joining of double-stranded oligonucleotide linkers or adaptors to DNA
• Site-directed mutagenesis
• Amplified fragment length polymorphism (AFLP)
• Ligase-mediated RNA detection (seeReference 3)
• Nick repair in duplex DNA, RNA or DNA/RNA hybrids
• Self-circularization of linear DNA.Notes
• Binding of T4 DNA Ligase to DNA may result in a band shift in agarose gels. To avoid this, incubate samples with 6X DNA Loading Dye & SDS Solution at 70°C for 5 min or 65°C for 10 minutes and chill on ice prior to electrophoresis.
• The volume of the ligation reaction mixture should not exceed 10% of the competent cell volume in the transformation process.
• Prior to electro-transformation, remove T4 DNA Ligase from the ligation mixture by spin column or chloroform extraction. The extracted DNA can be further precipitated with ethanol.

Specifications

General

Compatible Buffer	10X T4 DNA Ligase Buffer
Product Type	T4 DNA Ligase
Quantity	2 x 500 units
Concentration	1 U/μL
Enzyme	DNA Ligase
Unit Size	Each

Ordering

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Thermo Fisher, EL0016, T4 DNA Ligase, LC (1 U/μL)

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