Description
Quantity: 1,000 units
Unit Size: Each
• Source:E. colicells with a cloned gene 63 of bacteriophage T4
• Molecular Weight: 43.6 kDa monomer Quality Control:
• The absence of ribonucleases, exodeoxyribonucleases, endodeoxyribonucleases and phosphatases confirmed by appropriate quality tests. Source:
• E.colicells with a cloned gene 63 of bacteriophage T4Molecular Weight:
• 43.6kDa monomerDefinition of Activity Unit:
• One unit of the enzyme catalyzes the conversion of 1nmol of 5ft.-[^32P]-(A)_12-18to a phosphatase-resistant form in 30 min. at 37°C
• Enzyme activity is assayed in the following mixture: 50mM Tris-HCl (pH 7.5), 10mM MgCl_2, 10mM DTT, 1mM ATP, 10μM 5'-[^32P]-(A)_12-18(10μM in 5ft.-termini)Storage Buffer:
• The enzyme is supplied in:20mM Tris-HCl (pH 7.5), 1mM DTT, 50mM KCl, 0.1mM EDTA, 0.03% (v/v) ELUGENT Detergent and 50% (v/v) glycerol10X Reaction Buffer:
• 500mM Tris-HCl (pH 7.5 at 25°C), 100mM MgCl_2, 100mM DTT, 10 mM ATPInhibition and Inactivation:
• Inhibitors: metal chelators, SH group-modifying reagents (8).
• Inactivated by heating at 70°C for 10min. Recommended for:RNA 3'-end labeling with cytidine 3',5'-bis [alpha-^32P] phosphate (1); Joining RNA to RNA (2); Synthesis of oligoribonucleotides and oligodeoxyribonucleotides (3, 4); Specific modifications of tRNAs (5); Oligodeoxyribonucleotide ligation to single-stranded cDNAs for 5ft. RACE (Rapid Amplification of cDNA Ends) (6); Site-specific generation of composite primers for PCR (7) Note:The recommended BSA concentration in the reaction mixture is 0.1mg/ml.
