| Compatible Buffer | 10X Reaction Buffer |
|---|---|
| Product Type | RNase H |
| Quantity | 500 units |
| Concentration | 5 U/μL |
| Enzyme | RNase |
| Unit Size | Each |
Answer:It hydrolyzes the RNA strand in RNA–DNA hybrids rather than indiscriminately degrading free DNA or RNA.
Answer:500 units at 5 U/µL, corresponding to 100 µL of enzyme solution, with 10× reaction buffer.
Answer:Store at −20 °C and follow the supplier's handling instructions to maintain enzyme activity.
Thermo Scientific RNase H catalyzes hydrolysis of the RNA strand in RNA–DNA hybrids while leaving single-stranded or double-stranded DNA and RNA without the same substrate specificity. This enzyme is suited to molecular biology workflows such as removal of RNA following first-strand cDNA synthesis, preparation for second-strand synthesis and selected studies of hybrid nucleic acid structures. The enzyme is supplied at 5 U/µL with a total activity of 500 units, corresponding to 100 µL of enzyme solution. A 10× reaction buffer is included. Enzyme activity is defined using a hybrid-substrate assay, so reaction time and enzyme units should match the validated protocol instead of assuming complete RNA removal at any fixed dose. Keep reagents at −20 °C and work with RNase-free tubes and solutions. Set up the reaction with the recommended buffer, temperature and incubation time, then inactivate or remove the enzyme as required before downstream analysis. The product is for research use only and not for diagnostic procedures.
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
