Thermo Fisher, EN0521, DNase I, RNase-free (1 U/μL)

Name: DNase I, RNase-free (1 U/μL)
Brand: Thermo Fisher
SKU: EN0521

Quantity: 1, 000 units, Unit Size: Each, Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ions., • In the presence of Mg2+, DNase I cleaves each strand of dsDNA independently in a statistically random fashion, • In the presence of Mn2+, the enzyme

Catalog Number: EN0521

Pricing

Send an RFQ.

View RFQ List

Category

Phosphatase Inhibitors

Pack Size000 units / pack

TagsModifying Enzymes Enzymes and Inhibitors

CollectionsThermo Fisher Enzymes & Inhibitors for PCR, Cloning & Protein Protection Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 1,000 units
Unit Size: Each
Highlights
• Recombinant enzyme
• Purified from non-animal host with a lower level of intrinsic RNasesApplications
• Preparation of DNA-free RNA
• Removal of template DNA followingin vitrotranscription
• Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR
• DNA labeling by nick-translation in conjunction with DNA Polymerase I
• Studies of DNA-protein interactions by DNase I, RNase-free footprinting
• Generation of a library of randomly overlapping DNA inserts. Reaction buffer containing Mn2+ is usedNoteDNase I is sensitive to physical denaturation. Mix gently by inverting the tube. Do not vortex.

Specifications

General

Compatible Buffer	Reaction Buffer
Product Type	DNase I RNase Free
Quantity	1,000 units
Concentration	1 U/μL
Enzyme	DNase
Unit Size	Each

FAQs

Question: What is the supplied DNase I concentration?

Answer:The enzyme concentration is 1 unit per microliter, with 1,000 units supplied.

Question: Which nucleic acid does this enzyme remove?

Answer:DNase I digests single-stranded and double-stranded DNA while maintaining RNase-free processing conditions.

Question: Which downstream methods benefit from DNase treatment?

Answer:RNA preparation for reverse transcription, RT-PCR and RT-qPCR benefits from removal of contaminating genomic DNA.

Application Note

Thermo Scientific RNase-free DNase I degrades single-stranded and double-stranded DNA while protecting RNA-focused workflows from unwanted RNase activity. The enzyme is supplied at 1 U/µL with 1,000 units per package. DNase I requires calcium ions for activity and uses magnesium or manganese ions to support DNA cleavage. It removes genomic DNA from RNA preparations before reverse transcription, RT-PCR or RT-qPCR, and also supports removal of DNA templates after in vitro transcription. Gentle mixing preserves enzyme performance during reagent handling.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.