Thermo Fisher, EN0523, DNase I, RNase-free, HC (50 U/μL)

Name: DNase I, RNase-free, HC (50 U/μL)
Brand: Thermo Fisher
SKU: EN0523

Quantity: 1, 000 units, Unit Size: Each, Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ions., • In the presence of Mg2+, DNase I cleaves each strand of dsDNA independently in a statistically random fashion, • In the presence of Mn2+, the enzyme

Catalog Number: EN0523

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Category

Phosphatase Inhibitors

Pack Size000 units / pack

TagsModifying Enzymes Enzymes and Inhibitors

CollectionsThermo Fisher Enzymes & Inhibitors for PCR, Cloning & Protein Protection Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 1,000 units
Unit Size: Each
Highlights
• Recombinant enzyme
• Purified from non-animal host with a lower level of intrinsic RNasesApplications
• Preparation of DNA-free RNA
• Removal of template DNA followingin vitrotranscription
• Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR
• DNA labeling by nick-translation in conjunction with DNA Polymerase I
• Studies of DNA-protein interactions by DNase I, RNase-free footprinting
• Generation of a library of randomly overlapping DNA inserts. Reaction buffer containing Mn2+ is usedNoteDNase I is sensitive to physical denaturation. Mix gently by inverting the tube. Do not vortex.

Specifications

General

Compatible Buffer	Reaction Buffer
Product Type	DNase I RNase Free
Quantity	1,000 units
Concentration	50 U/μL
Enzyme	DNase
Unit Size	Each

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Thermo Fisher, EN0523, DNase I, RNase-free, HC (50 U/μL)

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General

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