Thermo Fisher, EN0591, Endonuclease IV, E.coli (2 U/μL)
Quantity: 100 units, Unit Size: Each, Endonuclease IV (Endo IV) recognizes apurinic/apyrimidinic (AP) sites of dsDNA and cleaves the phosphodiester bond 5' to the lesion generating a hydroxyl group at the 3'-terminus. The enzyme can also act as a 3'-diesterase that is able to release 3'-phosphoglycolate or 3'-phosphate from the damaged ends of dsDNA . Endo IV possesses also a 3'≥5' exonuclease activity. Its progression on substrates is sensitive to ionic strength, metal ions, EDTA, and reducing
Catalog Number: EN0591
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Pack Size100 units

