Thermo Fisher, ER0312, Eco47I (AvaII) (10 U/μL), 4,000 units

Eco47I (AvaII) is a type II restriction endonuclease used for sequence-specific cleavage of double-stranded DNA. The enzyme recognizes the degenerate sequence GGWCC, where W represents A or T, and cleaves within the recognition site to generate defined DNA fragments. This configuration provides 4000 units at a concentration of 10 U/µL. Optimal digestion is performed at 37 °C using a compatible restriction-enzyme buffer. Eco47I is an isoschizomer of AvaII and can be incorporated into cloning, restriction mapping, DNA construct verification and other workflows that require cleavage at GGWCC sites. Reaction design should account for the number of recognition sites, DNA quantity, buffer compatibility and any additional enzymes used in a double-digestion workflow. Complete digestion should be confirmed when fragment integrity is critical to downstream cloning or analytical interpretation. As with other restriction enzymes, methylation status and neighboring sequence context can affect practical digestion behavior and should be considered during assay design.