Thermo Fisher, ER0633, PvuII, HC (50 U/μL)

Name: PvuII, HC (50 U/μL)
Brand: Thermo Fisher
SKU: ER0633

Quantity: 12, 500 units, Unit Size: Each, Thermo Scientific™ PvuII is available as a FastDigest enzyme which is used for rapid DNA digestion, more than 90% of the digested DNA fragments can be ligated and more than 95% of these sites can be recut at a 5'-termini concentration of 0.15 μM after a 10-fold overdigestion (5U/μg DNA x 2 hours) with PvuII.

Catalog Number: ER0633

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Category

Restriction Enzymes

Pack Size500 units / pack

TagsRestriction Enzymes Enzymes and Inhibitors

CollectionsThermo Fisher Enzymes & Inhibitors for PCR, Cloning & Protein Protection Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 12,500 units
Unit Size: Each
5'...C A G▵C T G...3'3'...G T C▵G A C...5'Conditions for 100% Activity
• 1X Buffer G:10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl_2, 50mM NaCl and 0.1mg/mL BSA
• Incubate at 37°CStorage Buffer
• PvuII is supplied in:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerolLigation and Recleavage
• After 10-fold overdigestion with PvuII, more than 90% of the DNA fragments can be ligated and more than 95% of these can be recutMethylation Effects
• Dam: never overlaps —no effect
• Dcm: never overlaps —no effect
• CpG: never overlaps —no effect
• EcoKI: never overlaps —no effect
• EcoBI: may overlap —effect not determinedDigestion of Agarose-embedded DNA
• Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours Note:Greater than 15-fold overdigestion with PvuII may result in star activity.

Specifications

General

Compatible Buffer	10X Buffer G
Product Type	Restriction Enzyme
Quantity	12,500 units
Concentration	50 U/μL
Enzyme	Pvu II
Methylation Sensitivity	Not Dam Methylation-Sensitive
Optimal Reaction Temperature	37°C
Research Category	Traditional Cloning
Sensitive to Heat Inactivation	No
Type IIS RE	No
Unit Size	Each

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Thermo Fisher, ER0633, PvuII, HC (50 U/μL)

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