Thermo Fisher, FD0664, FastDigest SmaI, 200 μL (200 Reactions)

Name: FastDigest SmaI, 200 μL (200 Reactions)
Brand: Thermo Fisher
SKU: FD0664

Quantity: 200 μL (200 Reactions), Unit Size: Each, 5' CCC ↓ GGG 3'3' GGG ↑ CCC 5'FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.The universal buffer allows rapid single-, double- or multiple-DNA digestion within 5-15 minutes, eliminating any need for buffer change or subsequent DNA clean-up steps. DNA modifying enzymes, such as Klenow Fragment, T4 DNA ligase, alkaline phosphatases, and

Catalog Number: FD0664

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Category

Restriction Enzymes

Pack Size200 µL

TagsRestriction Enzymes Enzymes and Inhibitors

CollectionsThermo Fisher Enzymes & Inhibitors for PCR, Cloning & Protein Protection Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 200 μL (200 Reactions)
Unit Size: Each
Features
• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
• Direct loading on gels
• No star activity
• 176 FastDigest enzymes availableApplications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)
• SNP analysisNotes
• The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer.
• For applications that require product analysis by fluorescence excitation (e.g., concentration measurements in UV light), the colorless FastDigest Buffer is recommended.
• For methylation sensitivity, refer to product specifications.

Specifications

General

Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Inactivation Temperature	65°C
Overhang	Blunt
Product Type	Restriction Enzyme
Quantity	200 μL (200 Reactions)
Enzyme	Sma I
Methylation Sensitivity	CpG Methylation-Sensitive
Optimal Reaction Temperature	37°C
Product Line	FastDigest
Research Category	Traditional Cloning
Sensitive to Heat Inactivation	Yes
Type IIS RE	No
Unit Size	Each

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Thermo Fisher, FD0664, FastDigest SmaI, 200 μL (200 Reactions)

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General

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