Thermo Fisher, FD1254, FastDigest BcuI, 50 μL (50 reactions)

Name: FastDigest BcuI, 50 μL (50 reactions)
Brand: Thermo Fisher
SKU: FD1254

Quantity: 50 μL (50 reactions), Unit Size: Each, FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.The universal buffer allows rapid single-, double- or multiple DNA digestion within 5-15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% a

Catalog Number: FD1254

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Category

Restriction Enzymes

Pack Size50 µL

TagsRestriction Enzymes Enzymes and Inhibitors

CollectionsThermo Fisher Enzymes & Inhibitors for PCR, Cloning & Protein Protection Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 50 μL (50 reactions)
Unit Size: Each
Thermo Scientific FastDigest BcuI restriction enzyme recognizes A^CTAGT site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: SpeI, AhlI.Thermo Scientific FastDigest BcuI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment (Cat. No. EP0051), T4 DNA Ligase (Cat. No. EL0014), alkaline phosphatases and T4 DNA Polymerase (Cat. No. EP0061) all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.Features• 100% activity of all FastDigest enzymes in the universal buffer• 100% buffer compatibility with downstream applications• Complete digestion in 5–15 minutes• Direct loading on gels• No star activity• 176 FastDigest enzymes availableApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern Blot• Restriction fragment length polymorphism (RFLP)• SNP analysisNote: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.For methylation sensitivity, refer to product specifications.

Specifications

General

Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Product Type	Restriction Enzyme
Quantity	50 μL (50 reactions)
Enzyme	Bcu I
Methylation Sensitivity	Not Dcm Methylation-Sensitive
Optimal Reaction Temperature	37°C
Product Line	FastDigest
Research Category	Traditional Cloning
Sensitive to Heat Inactivation	No
Type IIS RE	No
Unit Size	Each

FAQs

Question: What recognition sequence is cleaved by FastDigest BcuI?

Answer:It recognizes A^CTAGT and produces 5′ protruding ends.

Question: What temperature is recommended for digestion?

Answer:The enzyme is used at 37°C.

Question: How many reactions are supplied?

Answer:This configuration provides approximately 50 reactions.

Application Note

FastDigest BcuI is used for rapid DNA restriction digestion in cloning and analytical workflows. The enzyme recognizes the sequence A^CTAGT and produces compatible 5′ protruding ends. BcuI is an isoschizomer of SpeI and AhlI, so it provides the same recognition and cleavage specificity for applications that use this six-base restriction site. Digestion is performed at 37°C and can generally be completed in 5–15 minutes with universal FastDigest or FastDigest Green Buffer. This configuration contains 50 µL of enzyme for approximately 50 reactions. The common buffer platform simplifies double and multiple digests because compatible FastDigest enzymes can be combined in the same reaction mixture. FastDigest Green Buffer can also be used when direct gel loading is convenient, while the colorless buffer is preferred for fluorescence-sensitive downstream measurements.

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