Thermo Fisher, J63135.AD, Denhardt's solution (50X)

Name: Denhardt's solution (50X)
Brand: Thermo Fisher
SKU: J63135.AD

Quantity: 50 mL, Unit Size: Each, Denhardt's solution is used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. Denhardt's Solution is a mixture of blocking agents used in membrane-based hybridization protocols. This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has

Catalog Number: J63135.AD

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Category

pH Standards

Pack Size50 mL

TagsBuffers and Standards General Chemistry Solutions

CollectionsThermo Fisher Buffers & Standards for pH, Conductivity, HPLC/IC/LC-MS Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 50 mL
Unit Size: Each
General Description
• Formulated in 1966, Denhardt's solution is a combination of high-molecular weight polymers used to prevent non-specific binding of nucleic acid probes to membranes and filters
• Denhardt's solution contains 1% Ficoll (type 400), 1% polyvinylpyrrolidone, and 1% bovine serum albuminApplication
• Denhardt's solution is used in Northern and Southern blotting to prevent non-specific binding of detection reagents to membranes
• It can be used along with gelatin-chrome alum in in situ hybridization assays to maintain chromosome integrity
• It has been used to facilitate quantitative DNA hybridization on nylon membranes

Specifications

General

Chemical Name or Material	Denhardt's solution
Concentration	50X
MDL Number	MFCD00717549
Physical Form	Liquid
Recommended Storage	Store at -20°C
Solubility Information	Fully miscible.
TSCA	Yes
Quantity	50 mL
Unit Size	Each

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Thermo Fisher, J63135.AD, Denhardt's solution (50X)

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