| Column Type | Spin Column |
|---|---|
| For Use With (Application) | PCR, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, In Vitro Transcription |
| High-throughput Compatibility | Compatible (Vacuum manifold) |
| Prep Scale | < 100 μg (Small-Scale) Plasmid DNA |
| Product Line | GeneJET |
| Product Type | Plasmid MiniPrep Kit |
| Quantity | 250 Preps |
| Sample Type | Bacteria |
| Scale | Mini |
| Target | Plasmid DNA |
| Test Time | <14 min |
| Format | Spin Column |
| Isolation Technology | Silica Column |
| Unit Size | Each |
Answer:The kit supports 250 plasmid DNA minipreparations.
Answer:It can recover up to 20 μg of high-copy plasmid DNA per isolation.
Answer:The starting sample is a bacterial culture.
GeneJET Plasmid Miniprep Kit is designed for purification of plasmid DNA from bacterial cultures through a rapid silica-membrane spin-column workflow. This package provides 250 plasmid minipreparations. Cells are resuspended and lysed, the lysate is neutralized and clarified, and plasmid DNA binds to the silica membrane under the appropriate salt conditions. After washing to remove proteins, salts and other contaminants, purified plasmid DNA is eluted in buffer or water. The kit can recover up to 20 μg of high-copy plasmid DNA per isolation, with a procedure typically completed in less than 14 minutes. Included reagents encompass resuspension, lysis, neutralization, concentrated wash solution, RNase A and elution buffer, together with spin columns and collection tubes. The recovered plasmid is suitable for cloning, restriction digestion, PCR, sequencing, transformation, labeling and in vitro transcription. The small-scale column format eliminates the need for phenol/chloroform extraction or conventional alcohol precipitation. This configuration is suited to repetitive molecular-cloning and plasmid-screening workflows where rapid, consistent recovery of usable DNA is required.
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