Thermo Fisher, K0692, GeneJET Gel Extraction Kit, 250 preps

Name: GeneJET Gel Extraction Kit, 250 preps
Brand: Thermo Fisher
SKU: K0692

Quantity: 250 Preps, Unit Size: Each

Catalog Number: K0692

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Category

Gel Extraction Kits

Pack Size250 preps

TagsMolecular Biology Reagents and Kits DNA Gel Extraction

CollectionsThermo Fisher Molecular Biology Reagents & Kits Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 250 Preps
Unit Size: Each
Thermo Scientific GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-melting point agarose gels run in either TAE or TBE buffer.The kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column. The kit can be used to purify DNA fragments from 25 bp to 20 kb in size with recovery rates up to 95%. Each GeneJET purification column has a binding capacity of up to 25 μg of DNA and can process up to 1 g of agarose gel. The entire procedure takes just 15 minutes, and the isolated DNA is ready to use in all common downstream applications, including ligation, restriction digestion, PCR, sequencing, and labeling.Highlights
• Highly efficient:up to 95% recoveries in the range of 100 bp to 10 kb
• Fast:procedure takes only 15 minutes
• Convenient:spin columns are capped and assembled with collection tubesApplicationsFast and efficient extraction of high purity DNA fragments ideal for use in all conventional molecular biology procedures including:
• FastDigest or conventional restriction digestion
• Automated fluorescent or radioactive sequencing
• PCR
• In situhybridization
• Labeling
• Cloning

Specifications

General

Column Type	Spin Column
Elution Volume	50 μL
Final Product Type	DNA
For Use With (Application)	PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
High-throughput Compatibility	Not High-throughput Compatible (Manual)
Quantity	250 Preps
Sample Type	Agarose gels
Shipping Condition	Room Temperature
Starting Material Amount	≤1 g
Test Time	15 min.
Yield	25 μg (Binding capacity)
Isolation Technology	Silica Spin Column
Unit Size	Each

FAQs

Question: What input sample does this kit process?

Answer:It purifies DNA from excised agarose gel fragments.

Question: What is the largest gel mass per column?

Answer:Each spin column can process up to 1 g of agarose gel.

Question: How many preparations are included?

Answer:The kit contains materials for 250 preparations.

Application Note

GeneJET Gel Extraction Kit, 250 preps is designed for purification of DNA bands excised from standard or low-melting-point agarose gels prepared in TAE or TBE buffer. It uses silica-membrane spin columns to bind DNA after the gel fragment has been dissolved, then washes away agarose and other impurities before elution. The kit purifies fragments from approximately 25 bp to 20 kb, with recovery of up to 95% and a column binding capacity of up to 25 μg of DNA. Each spin column can process as much as 1 g of agarose gel, and the typical workflow takes about 15 minutes. The supplied configuration includes materials for 250 preparations and allows elution into an approximately 50 μL volume. Recovered DNA is ready for PCR, restriction digestion, cloning, sequencing, labeling and other conventional molecular biology procedures. This kit is intended for DNA recovery from excised gel slices rather than routine cleanup of a PCR reaction directly from solution. The spin-column format avoids manual resin manipulation and produces a concentrated DNA solution after an easily repeatable centrifugation-based procedure.

Ordering

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