Description
Quantity: 50 Preps
Unit Size: Each
The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 15 minutes. The purified high quality RNA can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analysis.Highlights
• Efficient– up to 1.8 μg of total RNA from 500 μL of human blood
• Convenient– spin columns are capped and assembled with collection tubes
• Pure– RNA pure from enzyme inhibitors, proteins, ready to use in all demanding applications. Validated in cDNA synthesis and quantitative RT-PCR
• Fast and simple– procedure takes less than 15 minutes and does not involve organic extraction or differential RBC lysis
• Compatible– suitable for vide variety of samples - human and animal blood treated with EDTA, citrate or heparin, buffy coat, bone marrow, and related body fluidsApplications
• Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assaysncludes Lysis Buffer Wash Buffer WB 1 (concentrated) Wash Buffer 2 (concentrated) Water, nuclease-free GeneJET RNA Purification Columns (pre-assembled with collection tubes) Collection tubes, 2 mL Collection tubes, 1.5 mL Detailed Protocol