Thermo Fisher, K10201, Champion™ pET102 Directional TOPO™ Expression Kit with BL21 Star™ (DE3) One Shot™ Chemically Competent <i>E. coli</i>

Quantity: 20 reactions
Unit Size: Each
• Based on T7 expression vectors originally developed by Studier and colleagues, high-level expression is achieved because T7 RNA polymerase is more processive than nativeE. coliRNA polymerase and is dedicated to transcription of gene of interest
• Protein production is further enhanced in system by expression strain BL21 StarE. coli, which significantly improves stability of mRNA transcripts and increases protein expression up to ten-fold
• Simplified, efficient Directional TOPO cloning allows to quickly enter ChampionpET Expression vector
• Features linearized, topoisomerase I-activated ChampionpET expression vectors for five-minute directional cloning
• Directional TOPO Cloning technology facilitates gene expression becausa proofreading enzyme is used for PCR, resulting in fewer errors in cloned genes
• Greater than 90% of clones are in correct orientation for gene expression, reducing time spent on colony screening
• Each vector carries a T7lacpromoter for high-level expressionBacterial Expression/Protein Expression/Proteins, Expression, Isolation and Analysis