Thermo Fisher, K156002, PureLink™ FFPE RNA Isolation Kit

Quantity: 50 Preps
Unit Size: Each
The PureLink FFPE RNA Isolation Kit provides a simple, reliable, and rapid method to isolate high-quality total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue specimens for retrospective basic and clinical biological studies of gene expression without the use of toxic organic solvents for deparaffinization.Rapid and Non-Toxic Isolation ProcedureThe PureLink FFPE RNA Isolation Kit employs a simple heating procedure for deparaffinization, instead of relying on the use of toxic organic solvents such as xylene. RNA isolation can be completed in 30 to 40 minutes instead of days, as is required for older FFPE isolation methods. The heating procedure for deparaffinization partly restores RNA template structure for cDNA synthesis and improves template quality for reverse transcription.High Yield, High Quality RNAThe PureLink FFPE RNA Isolation Kit offers higher yields and purity as compared to other commercially available RNA purification systems, allowing for RT-PCR products over 1 kb in length. In addition, the PureLink FFPE RNA Isolation Kit minimizes genomic DNA contamination of the purified RNA sample and provides an option for DNase digestion.Purified Total RNA is Suitable for Use with a Variety of Downstream Applications and ProductsRNA extracted from FFPE specimens is suitable for downstream applications such as reverse transcription, real-time PCR (qPCR), real-time RT-PCR (qRT-PCR), and microarray analysis.
• Offers a rapid and environmentally friendly procedure for the extraction of RNA from FFPE tissue specimens
• Isolates RNA with maximum yield and minimum degradationNot high-throughput compatible (Manual)ApplicationsDNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from FFPE Samples