Description
Quantity: 960 Preps
Product Type: Buffers Only
Unit Size: Each
The mRNA Catcher PLUS Purification Kit offers:
• Robust performance—high binding capacity per well (>80 ng/well)
• Reproducible results—low CVs from well-to-well and plate-to-plate (∼2%)
• Optimized protocols—for small sample sizes (100 cells, 4 mg tissue, and 40 μL blood)
• Compatible plate design—may be used with most PCR cyclersHow it worksmRNA from total RNA, cells, tissue, and blood samples is hybridized to an immobilized, single-stranded 20-mer oligo(dT)-containing LNA. The incorporation of LNA into the oligo probe increases the hybridization efficiency and specificity of the mRNA binding. The mRNA can either be eluted or reversed-transcribed to cDNA directly in the well. The mRNA Catcher PLUS method does not require centrifugation, precipitation, phase separation, or column filtration. In addition, the entire method is amenable to automation for streamLined, high-throughput expression profiling for large-scale processes. Isolated mRNA is suitable for use in downstream applications such as RT-PCR and qRT-PCR.
