Thermo Fisher, K210004, PureLink™ HiPure Plasmid Midiprep Kit

Quantity: 25 Preps
Unit Size: Each
Advantages of the PureLink™ HiPure Plasmid Midiprep Kit include:
• Labor and time saving—no need for extra steps to remove endotoxin and other contaminants
• High-purity, low-endotoxin product—pure enough for mammalian cell transfection
• Versatility—purify any type and size of plasmid DNA, including BAC, bacmids, and ssM13 DNAsAnion-exchange chromatography for plasmid purificationThe PureLink™ HiPure Plasmid DNA Purification Kits use a patented anion-exchange resin to purify plasmid DNA to a level equivalent to two passes through CsCl gradients. The resin combines excellent capacity with a fast flow rate, high resolution, high yield, and efficient endotoxin removal. Plasmid preparations can typically be completed in less than 2 hours.Contaminant-free DNAUnlike cesium chloride gradient protocols, PureLink™ HiPure Plasmid Purification Systems do not use organic solvents, ethidium bromide, or cesium chloride, all of which are troublesome to work with and to dispose of. Typically, plasmid DNA prepared using the PureLink™ HiPure Plasmid Midiprep Kit will have an A_260/A_280 ratio of greater than 1.80, indicating that the DNA is reasonably free of proteins that might interfere with downstream applications. Endotoxin levels are typically within 0.1–1 EU/μg, making this plasmid DNA ideal for mammalian cell transfection.