Thermo Fisher, K990097, Expressway™ Maxi Cell-Free <i>E. coli</i> Expression System

Quantity: 1 Kit
Unit Size: Each
• Procedure can be performed in single reaction tube and is easily scalable without need for specialized equipment
• TOPO TA Cloning expression vectors (5-min cloning with 95% efficiency) are provided for optimal expression results
• In addition to all advantages of open expression system, produces high levels of recombinant protein that may be easily detected and purified for various downstream applications
• Proprietary formulation of Feed Buffer boosts reaction productivity so that more than one milligram of protein is produced in 2mL reaction within 4 to 6 hours
• The formulation of lysate enables protein synthesis using either circular or linear templates
• One kit is supports 200 x 50 reactions, which can be accommodated by 2 x 96-well plates
• Without feed buffer, such reactions need only 2 hours
• Once positive expression is detected, scale-up protein production can be performed with large cell-free format or inE. colicell-based systemsCell Free Protein Expression for Functional Analysis, Cell Free Protein Expression for Structural Analysis, Cell-Free (in vitro) Expression, Protein Expression, Proteins, Expression, Isolation and Analysis