Thermo Fisher, PQ1000101, ProQuest™ Two-Hybrid System with Gateway™ Technology

Quantity: 1 kit
Unit Size: Each
• Reconstitutes a functional transcription factor that activates chromosomally integrated reporter genes where transcriptional activation is monitored by the growth of cells on selective media
• Allow rapid and easy generation of bait and prey constructs, and facilitate downstream application
• Low copy-number DBD (DNA Binding Domain) and AD (Activation Domain) vectors (ARS/CEN) to control over-expression and increase reproducibility
• Three different reporter genes (HIS3,URA3, andlacZ) with independent promoter regions to rapidly weed out false positives
• URA3 reporter gene allows for both positive and negative selection, enabling advanced two-hybrid techniques such as reverse two-hybrid
• A set of strong-to-weak Gateway-based control interactions to evaluate results and assess the strength of the interaction being testedProtein-Protein Interactions, Proteins, Expression, Isolation and Analysis, Yeast Two-Hybrid