Thermo Fisher, Q10212, Qubit™ ssDNA Assay Kit

Name: Qubit™ ssDNA Assay Kit
Brand: Thermo Fisher
SKU: Q10212

Quantity: 1 kit, Unit Size: Each

Catalog Number: Q10212

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Category

Fluorescence Quantitation Kits

TagsMolecular Biology Reagents and Kits Nucleic Acid Labeling and Detection

CollectionsThermo Fisher Molecular Biology Reagents & Kits Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 1 kit
Unit Size: Each
The Qubit ssDNA Assay Kit is designed specifically for use with the Qubit Fluorometer, but may be used with any fluorometer or fluorescence plate reader. The Qubit™ ssDNA Kit is ideal for quantitating single-stranded DNA or oligonucleotides. However, it is not specific for single-stranded DNA. This assay kit will also detect double-stranded DNA and RNA but it will not detect contaminating protein or nucleotides. The assay is designed to be accurate for initial sample concentrations from 50 pg/μL to 200 ng/μL providing an assay range of 1–200 ng. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 μl and 20 μl is acceptable), and read the concentration using the Qubit Fluorometer. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay.Which product to choose for ssDNA quantitation?
• For 1–20 samples: use this Qubit ssDNA Assay Kit with the Qubit Fluorometer
• For 20–2000 samples: use the Quant-iT OliGreen ssDNA Assay Kit with microplate readerNotes:1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers.2. 500 μL thin-walled PCR tubes are required but not included.

Specifications

General

Assay	ssDNA Quantitation
Excitation/Emission	500/525
For Use With (Equipment)	Qubit Fluorometer
Product Line	Qubit
Quantitation Range	0.2 to 240 ng
Quantity	1 kit
Shipping Condition	Room temperature
Detection Method	Fluorescence
Unit Size	Each

FAQs

Question: What sample type is the assay designed to quantify?

Answer:It is designed primarily for single-stranded DNA and oligonucleotides.

Question: What initial concentration range is supported?

Answer:The assay is designed for approximately 50 pg/µL to 200 ng/µL initial sample concentration.

Question: What components are included?

Answer:The kit contains concentrated assay reagent, dilution buffer, and prediluted DNA standards.

Application Note

Qubit™ ssDNA Assay Kit provides fluorescence-based quantification of single-stranded DNA and oligonucleotides. The kit contains concentrated assay reagent, dilution buffer, and prediluted DNA standards and is designed for use with Qubit fluorometers, although the fluorescence chemistry can also be measured with compatible fluorometers or plate readers. The assay is accurate for initial sample concentrations of approximately 50 pg/µL to 200 ng/µL, corresponding to an assay range of about 1–200 ng. Common contaminants such as salts, solvents, detergents, proteins, and free nucleotides are generally well tolerated. Although optimized for ssDNA, the dye is not absolutely specific and can also respond to double-stranded DNA or RNA, so sample composition should be considered when interpreting results.

Ordering

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