Thermo Fisher, Q32854, Qubit™ dsDNA Quantification Assay Kits

Name: Qubit™ dsDNA Quantification Assay Kits
Brand: Thermo Fisher
SKU: Q32854

Quantity: 500 assays, Assay: dsDNA Quantitation, HS, Quantitation Range: 0.1 to 120 ng, Unit Size: Each, Achieve accurate and precise quantification of dsDNA with Qubit dsDNA BR (Broad Range) Assay Kits. These dsDNA quantification kits enable quick and selective detection of low and high abundance DNA samples, and can distinguish dsDNA from ssDNA, RNA, protein, and free nucleotides. Contaminants such as salts, solvents, or detergents are well-tolerated.

Catalog Number: Q32854

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Category

Fluorescence Quantitation Kits

Pack Size500 assays

TagsMolecular Biology Reagents and Kits Nucleic Acid Labeling and Detection

CollectionsThermo Fisher Molecular Biology Reagents & Kits Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 500 assays
Assay: dsDNA Quantitation, HS
Quantitation Range: 0.1 to 120 ng
Unit Size: Each
Qubit dsDNA HS and BR Assay Kits, designed for use with Qubit Fluorometers, are highly selective for double-stranded DNA (dsDNA) over single-stranded DNA (ssDNA), RNA, protein, and free nucleotides. All kits provide concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 μL and 20 μL is acceptable), and read the concentration using a Qubit Fluorometer.Qubit dsDNA HS Assay KitThe Qubit dsDNA HS (High Sensitivity) Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of sensitive DNA samples. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0.005 to 120 ng/μL, providing a detection range of 0.1-120 ng.Qubit dsDNA BR Assay KitThe Qubit dsDNA BR (Broad-Range) Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of DNA samples. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0.2 to 2,000 ng/μL, providing a detection range of 4-2,000 ng.Notes
• Qubit dsDNA HS and BR Assay kits can be used with any Qubit Fluorometer
• Use with thin-wall, clear, 0.5-mL PCR tubes (Cat. No. Q32856) for the Qubit 4 Fluorometer and 8 x 200-μL tube strips (Cat. No. Q33252) for the Qubit Flex Fluorometer

Specifications

General

Assay	dsDNA Quantitation, HS
Excitation/Emission	510/527
For Use With (Equipment)	Qubit Fluorometer
No. of Reactions	500 Reactions
Product Line	Qubit
Quantitation Range	0.1 to 120 ng
Quantity	500 assays
Shipping Condition	Room Temperature
Detection Method	Fluorescence
Unit Size	Each

FAQs

Question: How many assays are included?

Answer:This configuration supports 500 assays.

Question: What initial DNA concentration range is supported?

Answer:The assay is designed for approximately 5 pg/µL to 120 ng/µL.

Question: What nucleic acid does the assay preferentially quantify?

Answer:The chemistry is highly selective for double-stranded DNA over RNA.

Application Note

Qubit™ dsDNA High Sensitivity Assay Kit provides fluorescence-based quantification of double-stranded DNA with high selectivity over RNA. This configuration supports 500 assays and is designed for initial DNA concentrations of approximately 5 pg/µL to 120 ng/µL, corresponding to an assay range of about 0.1–120 ng depending on sample volume. The chemistry tolerates many common contaminants including salts, free nucleotides, solvents, detergents, and proteins. A small sample volume is mixed with working solution and measured on a compatible Qubit fluorometer. The kit is useful for low-concentration DNA quantification before sequencing, PCR, cloning, library preparation, and other workflows where absorbance measurements may not provide sufficient sensitivity or dsDNA selectivity.

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