Thermo Fisher, R0551, Glycogen, RNA grade

Name: Glycogen, RNA grade
Brand: Thermo Fisher
SKU: R0551

Quantity: 2 x 0.1 mL, Unit Size: Each, Glycogen is a highly purified polysaccharide derived from oysters. It is an inert carrier which significantly increases the recovery of nucleic acids by alcohol precipitation. Glycogen is insoluble in ethanol and forms a precipitate that traps target nucleic acids. During centrifugation, a visible pellet is formed which greatly facilitates handling of the precipitated nucleic acids. Glycogen quantitatively precipitates nucleic acids from diluted solutions w

Catalog Number: R0551

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General

Form	Liquid
Product Type	Glycogen
Quantity	2 x 0.1 mL
Unit Size	Each

FAQs

Question: What concentration is the RNA-grade glycogen solution?

Answer:The aqueous solution is supplied at 20 mg/mL.

Question: What is the main purpose of adding glycogen during nucleic-acid precipitation?

Answer:It acts as an inert carrier that improves recovery and helps form a visible pellet.

Question: How much reagent is supplied?

Answer:This configuration contains two 0.1 mL tubes.

Application Note

Glycogen, RNA grade is used as an inert carrier to improve recovery of small amounts of RNA or DNA during alcohol precipitation. The highly purified oyster-derived polysaccharide is supplied as a 20 mg/mL aqueous solution and is free of detectable host DNA and RNA contamination. Because glycogen is insoluble in ethanol, it co-precipitates with nucleic acids in the presence of salt and forms a visible pellet that is easier to handle during centrifugation and resuspension. The reagent is especially useful for dilute samples, short oligonucleotides and low nucleic-acid inputs where precipitation recovery can otherwise be poor. This configuration contains two 0.1 mL tubes. The RNA-grade formulation can be used for both RNA and DNA precipitation and is stored at -20°C.

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