Thermo Fisher, R0941, X-Gal Solution, ready-to-use

Name: X-Gal Solution, ready-to-use
Brand: Thermo Fisher
SKU: R0941

Quantity: 10 mL, Unit Size: Each, Formula: C_14H_15BrClNO_6 Molecular weight: 408.6X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate. Induction of the lacZ gene with IPTG leads to the hydrolysis of X-Gal and to the development of blue colonies (see Supporting data).X-Gal Solution, ready-to-use, is stable, 0.22 μm membr

Catalog Number: R0941

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Category

Molecular Identification Kits

Pack Size10 mL

TagsMolecular Biology Reagents and Kits Miscellaneous Molecular Biology Reagents

CollectionsThermo Fisher Molecular Biology Reagents & Kits Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 10 mL
Unit Size: Each
Induction of the lacZ gene with IPTG leads to the hydrolysis of X-Gal and to the development of blue colonies. X-Gal Solution, ready-to-use, is a stable, 0.22μm membrane filtered solution formulated for direct use in conjunction with IPTG for blue/white colony screening. Recommended for:Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies (1); Visualization of beta-galactosidase reporter gene expression in transfected eukaryotic cells; Detection of beta-galactosidase activity in immunological and histochemical procedures. Note:Preparation of a 20mg/mL stock solution in dimethylformamide (DMF) or dimethylsulfoxide (DMSO) is recommended for X-Gal. DMF dissolves some plastic materials. The direct addition of DMF-containing solution to plastic Petri dishes should be avoided. DMF is toxic. Allow X-Gal solution containing dimethylformamide to completely dry before plating bacteria on agar plates.

Specifications

General

Product Type	X-Gal Solution
Quantity	10 mL
Substrate Properties	Chemical Substrate
Substrate Type	Beta-Gal Substrate, X-Gal
Detection Method	Colorimetric
Unit Size	Each

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Thermo Fisher, R0941, X-Gal Solution, ready-to-use

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