Thermo Fisher, V102020, pcDNA™4/TO Mammalian Expression Vector

Quantity: 20 μg
Unit Size: 20 µg
• Uses repressor mechanism that blocks transcription from the powerful CMV promoter in absence of tetracycline
• Because the T-REx System elements do not use viral transactivators, user can achieve high-level expression from complete CMV promoter without secondary, nonspecific activation of host genes
• Two tetracycline operator sequences (TetO_2) have been inserted between TATA box of the CMV promoter and the transcriptional start site: The TetO_2sequence itself has no effect on expression When tetracycline repressor protein (TR) is present, it effectively binds TetO_2sites and blocks transcription initiation
• Tetracycline added to culture medium binds to, and changes conformation of, TR protein
• This change causes TR protein to release TetO_2sites, derepressing transcription from the CMV promoter for high-level expression of gene of interest
• Expression levels can be modulated based on tetracycline concentration and can be induced to levels that are achieved with constitutive CMV expression vectors
• Zeocin or hygromycin resistance gene for effective selection of stable mammalian cell lines
• Large multiple cloning site to simplify cloningMammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Regulated Expression, Targeted Integration