Thermo Fisher, V17320, pPIC3.5K <i>Pichia</i> Vector

Name: pPIC3.5K Pichia Vector
Brand: Thermo Fisher
SKU: V17320

Quantity: 20 μg, Unit Size: 20 µg

Catalog Number: V17320

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Category

Molecular Identification Kits

Pack Size20 µg

TagsMolecular Biology Reagents and Kits DNA Vectors

CollectionsThermo Fisher Molecular Biology Reagents & Kits Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 20 μg
Unit Size: 20 µg
The pPIC3.5K vector carries the kanamycin resistance gene that confers resistance to Geneticin™ Reagent in Pichia. Spontaneous generation of multiple insertion events can be identified by resistance to increased levels of Geneticin™ Reagent. Pichia transformants are selected on histidine-deficient medium and screened for their level of resistance to Geneticin™ Reagent. The ability to grow in high concentrations of Geneticin™ indicates that multiple copies of the kanamycin resistance gene and the gene of interest are integrated into the genome. The pPIC3.5K vector directs secretion of proteins that are not expressed, but remain intracellular. This vector is included in the Multi-Copy Pichia Expression Kit (Cat. No. K175001).

Specifications

General

Product Type	Pichia Expression Vector
Quantity	20 μg
Promoter	AOX1
Protein Tag	Untagged
Unit Size	20 µg

Ordering

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Thermo Fisher, V17320, pPIC3.5K <i>Pichia</i> Vector

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General

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