Thermo Fisher, V19020, pPICZ A, B, & C <i>Pichia</i> Vectors
Quantity: 20 μg, Unit Size: 20 µg
Catalog Number: V19020
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Category
Pack Size20 µg
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| Antibiotic Resistance Bacterial | Zeocin™ (ZeoR) |
|---|---|
| Product Type | Pichia Expression Vector |
| Quantity | 20 μg |
| Vector | pPIC |
| Cloning Method | Restriction Enzyme/MCS |
| Promoter | AOX1 |
| Protein Tag | His Tag (6x) |
| Unit Size | 20 µg |
Answer:The vectors use the methanol-inducible AOX1 promoter.
Answer:The vectors carry Zeocin resistance for selection in Pichia and bacteria.
Answer:The vectors provide optional C-terminal c-Myc and 6xHis tags when the insert is cloned in frame.
pPICZ A, B and C Pichia Vectors are used to construct methanol-inducible recombinant protein expression strains in Pichia pastoris. The three vector variants provide different reading frames around the multiple-cloning region, allowing the inserted coding sequence to be aligned with optional C-terminal detection and purification tags. Expression is driven by the AOX1 promoter, while the Zeocin™ resistance gene supports direct selection of transformed Pichia cells and can be used to enrich for multi-copy genomic integrants by increasing selective pressure. The vectors provide a c-Myc epitope and a 6xHis tag for detection or immobilized-metal affinity purification when the insert is cloned appropriately. This package contains 20 µg each of pPICZ A, B and C in TE buffer and also includes a GS115/pPICZ/lacZ positive-control strain.
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