Thermo Fisher, V35120, pRSET A, B, & C Bacterial Expression Vectors

Quantity: 20 μg
Unit Size: 20 µg
• Expression is induced by production of T7 RNA polymerase in BL21(DE3)E. coli
• These cells also produce T7 lysozyme to reduce basal expression of target genes
• pRSET vector offers bacteriophage T7 promoter for high-level expression
• T7 gene 10 sequence to provide protein stability
• N-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-HisG Antibody
• N-terminal Xpress epitope for detection with the Anti-Xpress Antibody
• Enterokinase cleavage site for removal of fusion tag
• Each of three vectors provided (A, B, and C) has N-terminal tag coding sequence in a different reading frame relative to multiple cloning site to simplify in-frame cloning of the geneBacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis