Thermo Fisher, V45001, pBAD/gIII Kit

Name: pBAD/gIII Kit
Brand: Thermo Fisher
SKU: V45001

Quantity: 1 kit, Unit Size: 1 kit

Catalog Number: V45001

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Category

Molecular Identification Kits

TagsMolecular Biology Reagents and Kits DNA Vectors

CollectionsThermo Fisher Molecular Biology Reagents & Kits Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 1 kit
Unit Size: 1 kit
• To simplify purification, vector pBAD/gIII encodes a leader peptide which directs recombinant protein into the periplasmic space
• FeaturesaraBADpromoter for tightly regulated expression
• Translation initiation signals for optimizedE. coliexpression
• Leader peptide from bacteriophage fd gene III protein (gIII) for secreted expression
• C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin
• C-terminalc-mycepitope for detection and analysis with an Anti-mycAntibody
• A set of three vectors is provided (A, B, and C): Each has the C-terminal tag in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of the geneBacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

General

Antibiotic Resistance Bacterial	Ampicillin (AmpR)
Constitutive or Inducible System	Inducible
Inducing Agent	Arabinose
Product Type	Bacterial Expression Vector
Quantity	1 kit
Selection Agent (Eukaryotic)	None
Vector	pBAD
Cloning Method	Restriction Enzyme/MCS
Promoter	araBAD
Protein Tag	c-Myc Epitope Tag
Unit Size	1 kit

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Thermo Fisher, V45001, pBAD/gIII Kit

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General

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