Thermo Fisher, V600520, pOG44 Flp-Recombinase Expression Vector

pOG44 is a 5.8 kb helper plasmid for the Flp-In™ targeted-integration system. The vector constitutively expresses Flp recombinase from the human CMV immediate-early promoter. When pOG44 is cotransfected with an FRT-containing Flp-In expression plasmid into a compatible host cell line, the transiently expressed recombinase mediates site-specific integration at the genomic FRT site. The construct includes a synthetic intron to enhance FLP expression and does not contain a mammalian antibiotic-resistance marker for stable selection, helping keep selection focused on the integrated expression vector. This configuration contains 20 µg of plasmid DNA. The system is useful for generating isogenic mammalian cell lines with reproducible insertion of a gene of interest at a defined genomic location. Store the lyophilized vector at −20 °C after receipt and resuspend using sterile nuclease-free conditions.