Thermo Fisher, V66020, pDisplay™ Mammalian Expression Vector

Quantity: 20 μg
Unit Size: 20 µg
• Displayed proteins can be analyzed for their ability to interact with known or putative ligands
• Proteins of interest are targeted and anchored to cell surface by cloning the gene of interest in frame with vectors unique N-terminal secretion signal and C-terminal transmembrane anchoring domain of platelet-derived growth factor receptor (PDGFR)
• In contrast to phage display vectors which operate exclusively in prokaryotic cells, pDisplay vector offers advantage of having displayed protein of interest processed in mammalian cells
• Therefore, recombinant proteins of eukaryotic origin that are expressed from pDisplay more closely resemble their native form: this may favor more accurate ligand binding interactions
• In addition to N-terminal cell surface targeting signal and C-terminal transmembrane anchoring domain, pDisplay vector contains T7 promoter/priming site forin vitrotranscription of sense RNA and for sequencing of inserts
• Neomycin resistance marker for stable selection in mammalian cells using Geneticin™
• SV40 origin for replication and simple vector rescue in cell lines expressing large T antigen (e.g.,COS-1 and COS-7)
• Ampicillin resistance gene for selection inE. coliMammalian Two-Hybrid, Protein-Protein Interactions, Proteins, Expression, Isolation and Analysis