Thermo Fisher, V79020, pcDNA™3.1⁽⁺⁾ Mammalian Expression Vector

pcDNA™3.1(+) is an untagged mammalian expression vector designed for high-level constitutive expression of cloned genes. The vector uses the human CMV enhancer-promoter, includes a forward-orientation multiple cloning site, and carries a Geneticin™ resistance marker for selection of stable mammalian transfectants. Additional elements include the bovine growth hormone polyadenylation signal, SV40 origin, ampicillin-resistance gene, and pUC origin for plasmid propagation in E. coli. This configuration contains 20 µg of supercoiled, lyophilized plasmid together with an expression control. The (+) orientation refers to the direction of the multiple cloning site relative to the promoter and should be considered when designing restriction-enzyme cloning strategies. The vector is suitable for transient or stable expression in a wide range of mammalian cell lines when the insert includes an appropriate coding sequence and translation-initiation context.