General descriptionMethylation of cytosines located 5′ to guanosine is known to have a profound effect on the expression of many eukaryotic genes. In normal cells methylation occurs predominantly in CG-poor regions, while CG-rich areas, called CpG-islands remain unmethylated. The exceptions are the extensive methylation of CpG islands associated with transcriptional inactivation of regulatory regions of imprinted genes and genes on the inactive X-chromosome of females. Aberrant methylation of normally unmethylated CpG islands has been documented as a relatively frequent event in immortalized and transformed cells and has been associated with transcriptional inactivation of defined tumor suppressor genes in human cancers. Hundreds of CpG islands are now known to exhibit the characteristic of hypermethylation in tumors resulting in identification of candidate genes that can be interrogated to determine extent of tumor specific transformation.
ApplicationFor MSP primer design, please use the
MethPrime software package. Click hereOther Notes2X Extraction Buffer Modification Reagent Conversion Buffer Resuspension Buffer Equilibration Buffer Binding Buffer Wash Buffer I Wash Buffer II Elution Buffer Proteinase K Proteinase K Storage Buffer Conversion Plates/Cover films Desalting Plates Collection Plates Elution Plates
DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
