Merck, 17-294, Rho Activation Assay Kit

General description
A GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E. coli. Provided bound to glutathione-agarose. Purity >90%.
Application
PP1/PP2A Toolbox for the selective in vitro dephosphorylation of proteins.
Packaging
Kit capacity: 30 assays
Analysis Note
Routinely evaluated by precipitating GTP- Rho from 3T3/A31 cell lysates, subsequent detection by immunoblot analysis using anti-Rho (05-778).
Other Notes
100X GTPγS, 10mM (Cat.# 20-176)

100X GDP, 100mM (Cat.# 20-177)

Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)

Anti-Rho (-A, -B, -C), clone 55 (Cat.# 05-778)

Rho Assay Reagent (Rhotekin RBD, agarose) (Cat.# 14-383)
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.