General descriptionChromatin Immunoprecipitation (ChIP) is an important technique allowing the researcher to analyze in vivo interactions of proteins with genomic DNA. Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists. One can measure different proteins localized to a specific region of the genome, or the genome wide distribution of a specific protein. Another powerful application of this technique is to analyze changes in histone modifications that correlate with processes like transcription, mitosis or DNA repair.
Historically, ChIP protocols have been geared towards homogenous populations of cell culture. In contrast, tissue samples are more complex and the cells are heterogeneous thus making ChIP analysis less amenable. The Magna ChIP G Tissue Kit provides the tools necessary to obtain repeatable, reliable, and site specific tissue biopsies. Microdissected functionally related populations of cells within a heterogeneous tissue can thus be analyzed with ease, precision and certainty.
ApplicationThe Magna ChIP G Tissue Kit provides the tools necessary to obtain repeatable, reliable & site specific tissue biopsies.
Packaging22 ChIP assays from tissue samples.
Two modules containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions from tissue samples.
Preparation NoteUpon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when used and stored as directed.
Other Notes- Magnetic Protein G beads
- ChIP Dilution Buffer
- Low Salt Wash Buffer
- High Salt Wash Buffer
- LiCl Wash Buffer
- TE Buffer
- Tissue Lysis Buffer
- 10X Tissue Stabililizing Solution
- ChIP Elution buffer (w/o Proteinase K)
- 10X Glycine
- 10X PBS
- 1mm Micro-dissection punch
- Spin Filters
- Collection Tubes
- Bind Reagent A
- Wash Reagent B
- Elution Reagent C
- Protease Inhibitor Cocktail II
- Proteinase K
Legal InformationMAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
