Description
General description
Phosphorylation of the histone variant H2A.X is a rapid and sensitive response to double strand DNA breaks. The H2A.X Phosphorylation Assay Kit (Chemiluminescence Detection) is a cell based assay formatted for microplate-based detection of levels of phosphorylated histone H2A.X.
Application
The H2A.X Phosphorylation, Chemiluminescence Detection Assay Kit is a cell-based ELISA formatted for chemiluminescent detection of relative levels of phosphorylated H2A.X in microplate cells cultures.
Analysis Note
The H2A.X Phosphorylation, Chemiluminescence Detection Assay Kit is a cell-based ELISA formatted for chemiluminescent detection of relative levels of phosphorylated H2A.X in microplate cells cultures. Adherent cells are cultured in microplates, treated with agents that induce DNA damage/H2A.X phosphorylation, and are then fixed and permeabilized. Histone H2A.X phosphorylated at serine 139 is detected by Anti-phospho-H2A.X (Ser139), clone JBW301 and an anti-mouse-HRP (horseradish peroxidase) conjugate. The chemiluminescent HRP substrate LumiGLO is then added, and signal is measured in a microplate luminometer
Other Notes
Anti-phospho-Histone H2A.X (Ser139), clone JBW301 (Cat.# 05-636)
TBS, 20X (Cat.# 20-190)
20% Tween-20 (v/v)
10% BSA in TBS (Blocking Buffer)
LumiGLO Substrate Reagent A
LumiGLO Substrate Reagent B
Goat Anti-Mouse IgG, HRP conjugate
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
