Description
General description
Recombinant protein corresponding to human PCAF (a.a. 782-832).
~93 kDa
Application
Epitope: Located between amino acids 782-832
Biochem/physiol Actions
Recognizes PCAF in regions between a.a. 782-832.
Packaging
25 assays per set. Recommended use: 0.7 μg of antibody per chromatin immunoprecipitation (dependent upon biological context).
Physical form
Anti-PCAF (Rabbit Polyclonal), Part No. CS216159. One vial containing 17.5 µg purified rabbit polyclonal in 25 µL buffer containing Tris-citrate/phosphate buffer (pH 7 to 8) and 0.09% sodium azide, before the addition of 30% glycerol (0.7 mg/mL final). Store at -20°C.
Normal Rabbit IgG, Part No. PP64B. One vial containing 125 μg of purified Rabbit IgG in 125 μL storage buffer containing 0.05% sodium azide. Store at -20°C.
ChIP Primers, PRAME. Part No. CS216150. One vial containing 75 μL of 5 μM of each primer specific for human preferentially expressed antigen in melanoma (chr22:22901678+22901804, hg19 build). Store at -20°C.
FOR: GAA GCC TCG CCG GAA CTC
REV: TCC TCC CCA TCT CTG CAG AA
Preparation Note
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from K562 cells fixed for 10 min (4e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 0.7 µg of either Normal Rabbit IgG (Part No. PP64B), or 0.7 µg Anti-PCAF (Part No. CS216159) and the Magna ChIP HiSens Kit (Cat. # 17-10460). Successful immunoprecipitation of PCAF associated DNA fragments was verified by qPCR using ChIP Primers, PRAME (Part No. CS216150).
Please refer to the MagnaChIP HiSens (Cat. # 17-10460) or EZ-MagnaChIP HiSens (Cat. # 17-10461) protocol for experimental details.
Other Notes
Demonstrated to react with human. May react with additional species due to sequence homology.
Includes normal rabbit IgG and primers specific for the human PRAME.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
