Description
General description
This panel focuses on phosphorylated proteins within the MAPK and SAPK pathways, which regulate cellular responses to stress, growth factors, and cytokines. ERK1/2 (pT185/pY187), JNK (pT183/pY185), and p38 (pT180/pY182) are core MAPKs involved in proliferation, apoptosis, and inflammation. MEK1 (pS217/pS221) activates ERK, while MSK1 (pS376) and RSK1 (pS380) are downstream effectors. c-Jun (pS73) and ATF2 (pT71) are transcription factors regulated by JNK and p38. HSP27 (pS82) and MKK3/6 (pS189/pS207) are involved in stress response and MAPK activation.
Application
MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze 10 analytes in cell lysate samples.
Analytes Included: ERK1/2 (pT185/pY187), JNK (pT183/pY185), p38 (pT180/pY182), MEK1 (pS217/pS221), MSK1 (pS376), RSK1 (pS380), c-Jun (pS73), ATF2 (pT71), HSP27 (pS82), MKK3/6 (pS189/pS207)
Assay Characteristics: Refer to the assay protocol for details on cross-reactivity, buffer compatibility, lysate control, and recommended loading control (MAPmate™).
Features and Benefits
Simultaneously detect 10 phosphorylated proteins in MAPK and SAPK signaling pathways
Includes ERK, JNK, p38, MEK1, MSK1, RSK1, c-Jun, ATF2, HSP27, and MKK3/6
Magnetic bead-based format ensures reproducibility and efficient processing
Compatible with cell lysates from stress response, apoptosis, and inflammation studies
Ideal for toxicology, oncology, and cell biology research
Fast, convenient alternative to Western Blotting and immunoprecipitation procedures
Legal Information
Luminex is a registered trademark of Luminex Corp
MAPMATE is a trademark of Merck KGaA, Darmstadt, Germany
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
For research use only. Not for use in diagnostic procedures.
Label License/Sticker for Assay Product:
By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
