Description
General description
This panel targets phosphorylated and total Akt, a central kinase in the PI3K/Akt pathway. Akt (pS473) is activated in response to growth factors and insulin, promoting cell survival and metabolism. Measuring both phosphorylated and total Akt provides insight into pathway activation and protein expression levels in disease models.
Application
MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze 2 analytes in human, mouse, or rat cell lysate samples.
Analytes Included: Akt (Phospho-Ser473), Akt (Total)
Assay Characteristics: Refer to the assay protocol for details on cross-reactivity, buffer compatibility, lysate control, and recommended loading control (MAPmate™ Control Assays).
Features and Benefits
Simultaneously detect phosphorylated and total Akt in a single well
Includes Akt (Phospho-Ser473) and Akt (Total)
Magnetic bead-based format ensures reproducibility and efficient processing
Compatible with cell lysates from cancer, insulin signaling, and survival pathway studies
Ideal for oncology, metabolism, and signal transduction research
Fast, convenient alternative to Western Blotting and immunoprecipitation procedures
Legal Information
Luminex is a registered trademark of Luminex Corp
MAPMATE is a trademark of Merck KGaA, Darmstadt, Germany
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
For research use only. Not for use in diagnostic procedures.
Label License/Sticker for Assay Product:
By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
