Description
General description
This panel includes total proteins from multiple signaling pathways that regulate cell survival, proliferation, inflammation, and transcriptional activity. ERK1/2, JNK, and p38 are core components of the MAPK pathway involved in stress and growth responses. Akt regulates cell survival and metabolism. NFκB controls inflammatory and immune signaling. STAT3 and STAT5A/B mediate cytokine-driven transcriptional regulation. CREB is a transcription factor activated by cAMP signaling, and p70 S6 Kinase plays a role in protein synthesis and cell growth. Quantifying these total proteins supports pathway profiling and comparative analysis across experimental conditions.
Application
MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze 9 analytes in human, mouse, or rat cell lysate samples.
Analytes Included: ERK/MAPK 1/2, Akt, STAT3, JNK, p70 S6 Kinase, NFκB, STAT5A/B, CREB, p38
Assay Characteristics: Refer to the assay protocol for details on cross-reactivity, buffer compatibility, lysate control, and recommended loading control (MAPmate™ Control Assays).
Features and Benefits
Simultaneously detect 9 total proteins across key signaling pathways
Covers MAPK, STAT, Akt, NFκB, and CREB pathways for broad cellular profiling
Magnetic bead-based format ensures reproducibility and efficient processing
Compatible with cell lysates from diverse experimental conditions
Ideal for signal transduction, oncology, immunology, and toxicology research
Fast, convenient alternative to Western blotting and immunoprecipitation procedures
Legal Information
Luminex is a registered trademark of Luminex Corp
MAPMATE is a trademark of Merck KGaA, Darmstadt, Germany
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
For research use only. Not for use in diagnostic procedures.
Label License/Sticker for Assay Product:
By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
